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SDS-PAGE of the active and inactive forms of TSHR-289. Affinity-purified forms of TSHR-289 (19) were subjected to 12% SDS-PAGE before or after reduction (100mM dithiothreitol for 45 minutes at 50°C) followed by Coomassie blue staining. The broad bands reflect the very large degree of glycosylation. Without reduction, higher-order forms of TSHR-289 are evident, to a greater extent in inactive (In-Act) than in active (Act) molecules.
