Table 2.
Real-time iq-PCR quantification of aflatoxin in animal feeds, corn feed grain, and yellow corn meal samples. The calculated and dilution-corrected aflatoxin (μg/kg) concentrations are shown for the undiluted and diluted methanol/water extracts of un-spiked and spiked samples.
| Methanol/water (60:40) extracts | Cycle threshold (Ct) number 1 | Calculated aflatoxin (μg/kg) | Dilution-corrected aflatoxin (μg/kg) |
|---|---|---|---|
| Dairy feed 1 | 25.74 a | 4.18 | |
| Dairy feed 1 (1:10) | 29.38 b | 0.34 | 3.38 |
| Dairy feed 2 | 29.44 a | 0.32 | |
| Dairy feed 2 (1:10) | 30.57 b | 0.15 | 1.48 |
| Horse feed | 28.33 a | 0.70 | |
| Horse feed (1:10) | 32.46 b | 0.04 | 0.40 |
| Poultry feed | 28.78 a | 0.51 | |
| Poultry feed (1:10) | 28.32 a | 0.70 | 7.03 |
| Corn feed grains | 29.06 a | 0.42 | |
| Corn feed grains (1:10) | 27.11 b | 1.62 | 16.23 |
| Yellow corn meal | 28.51 a | 0.62 | |
| Yellow corn meal (1:10) | 26.52 b | 2.44 | 24.40 |
| Dairy feed 1 + AFB1 | 28.81 a | 0.50 | |
| Dairy feed 1 + AFB1 (1:10) | 27.95 b | 0.91 | 9.08 |
| Dairy feed 1 + AFB1 (1:40) | 30.1 c | 0.21 | 8.21 |
| Poultry feed + AFB1 | 29.61 a | 0.29 | |
| Poultry feed + AFB1 (1:10) | 26.97 b | 1.79 | 17.88 |
| Poultry feed + AFB1 (1:40) | 28.96 c | 0.45 | 18.06 |
| Horse feed + AFB1 | 29.63 a | 0.28 | |
| Horse feed + AFB1 (1:10) | 29.81 a | 0.25 | 2.51 |
| Horse feed + AFB1 (1:40) | 25.53 b | 4.84 | 193.53 |
| Yellow corn meal + AFB1 | 27.92 a | 0.93 | |
| Yellow corn meal + AFB1 (1:10) | 29.51 b | 0.31 | 3.09 |
| Yellow corn meal + AFB1 (1:40) | 29.41 b | 0.33 | 13.23 |
1 Data within the same feed set with different lower case letters are significantly different (p < 0.05).