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. 2014 Dec 16;6(12):3406–3425. doi: 10.3390/toxins6123406

Figure 4.

Figure 4

Anisomycin induces transient PKR activation in HeLa cell-free system. (A) Anisomycin (10 to 50 ng/mL), poly (IC) (100 ng/mL) and/or PKR inhibitors, 2-AP (2 mM) and C16 (2 µM), were added to HeLa-based cell-free assay mixtures. After incubation for 20 min at 30 °C, Western analysis was conducted with PKR and p-PKR antibodies; (B) HeLa-based cell-free assay mixtures were incubated with anisomycin (20 ng/mL) for indicated time intervals and subjected to Western blotting analysis with PKR, p-PKR and eIF2α, p-eIF2α antibodies. Data are representative of at least three replicate experiments.