Figure 7.

Distribution of RNA-IP-identified PKR-associated sequences in HeLa-based cell-free assay mixtures. HeLa lysate assay mixtures were incubated with or without DON (250 ng/mL) at 30 °C for 20 min and subjected to RNA-IP using PKR-specific antibody in four independent experiments. Following purification, cloning and sequencing of immunoprecipitated PKR-associated rRNAs, sequences were aligned to human 18S (NCBI Reference Sequence: NR-003286.2) and 28S (NCBI Reference Sequence: NR_03287.2) ribosomal RNA genes and their relative sizes and locations depicted above. The upper (solid) and lower (dotted) lines indicate the PKR-associated sequences identified from DON-treated and control, respectively. Integers in parentheses denote the number of clones recovered that were recovered in designated region.