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DON does not alter PKR binding to rRNA in HeLa-based cell-free assay mixtures. (A) Sequences and sizes of 32P labeled probes for 18S-U1 28S-U2 and 28S-C1; (B) Radiolabeled probes were incubated with immunoprecipitated RNAs from control and DON-treated HeLa extracts, subjected to RNase treatment, separated by urea-PAGE and analyzed by autoradiography.
