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. 2014 Dec 8;111(51):18303–18308. doi: 10.1073/pnas.1421815112

Fig. 4.

Fig. 4.

Immunohistochemical studies of GH, GHR, and IGF1 in ocular tissues. (A) In normal rats, no GH (red) was detected in the iris and ciliary body. (B) Double-labeling in normal eye showed GHR (green) in the ciliary epithelium (arrow); IGF1 (red) was not detected. (C) Twenty-four hours after LPS injection, GH was found in the epithelial cells lining the ciliary body and iris (orange arrow) and in the protein exudates in the anterior and posterior chambers (white arrow). (D) LPS triggered up-regulation of GHR (green) on the ciliary epithelium (orange arrow) and secretion of IGF1 (white arrow) in the anterior and posterior chambers. (E–G) The infiltrating cells in the anterior chamber were strongly immunoreactive for GH (orange arrow in E), GHR (arrows in F), and IGF1 (orange arrow in G). GH and IGF1 also were found in the protein exudation in the anterior chamber. (Scale bars: 35 µm.)