Skip to main content
. 2014 Nov 24;111(51):18309–18314. doi: 10.1073/pnas.1412172111

Fig. 5.

Fig. 5.

Endothelial ELAVL1 regulates postnatal pathological angiogenesis. Revascularization of the mouse ischemic hind limb was conducted as described in WT and Elavl1 ECKO mice. (A) Representative laser Doppler flow images of hind limb perfusion before (pre) and at different time points (post 3, 7, 10, and 14 d) after femoral artery ligation (R, control; L, ligated). (B) Changes in perfusion are shown as ratio of ischemic to control limb flow perfusion (n = 7 per group, *P < 0.05, ***P < 0.001). (C) Micro-CT reconstruction of a representative mouse hind limb 14 d after femoral artery ligation. Quantitative analysis of micro-CT angiograms in the calf is presented as total number of vascular structures of specified diameter. (n = 7 per group, *P < 0.05) (D) Representative images of CD31 immunostained sections of ligated limb muscle and quantification of CD31 positive vessels. The number of capillaries per field (20× WT: 91.1 ± 10.9 vs. Elavl1 ECKO: 35.5 ± 4.4, P = 0.0002, n = 8 images from four mice per group). The number of capillaries per fiber (WT: 3.57 ± 0.11 vs. Elavl1 ECKO 1.55 ± 0.06, P < 0.0001, n = 101 fibers from eight images per group). (Scale bar, 50 µm.)