Skip to main content
. 2014 Dec 15;111(51):E5564–E5573. doi: 10.1073/pnas.1419260111

Fig. 4.

Fig. 4.

PI3K/mTOR identified as essential by in vitro and in vivo functional genomics screening. (A) Schematic of experimental design of genome-wide shRNA screen in mouse osteosarcoma (mOS) cell line. (B) Venn diagram outlining the overlap of genes identified by two methods of analysis: weighted second best and Kolmogorov–Smirnov statistic in GENE-E/RIGER. (C) The relative viability of mOS cell lines, determined by Alamar Blue assay, expressing four individual shRNAs each targeting Mtor, Pik3ca, or control shRNAs (GFP, LacZ, Luciferase, and RFP) compared with uninfected cells that were puromycin selected for 8 d (empty). Horizontal bars represent mean and SEM, n = 3. (D) Mean log-twofold change in shRNA abundance of control (GFP, LacZ, Luciferase, and RFP) or experimental shRNAs from five tumor samples compared with preinjection samples. *P < 0.01. (E) The relative viability of mOS cell lines expressing shRNAs targeting either Pik3ca, Pik3cb, or control (luciferase) at 9 d postinfection and puromycin selection determined by WST-1assay. Error bars are SEM, n = 3.