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. 2014 Dec 15;111(51):E5564–E5573. doi: 10.1073/pnas.1419260111

Fig. 5.

Fig. 5.

Small-molecule inhibitors of PI3K/mTOR inhibit osteosarcoma proliferation. Human OS cell lines (MG63, U2OS, SJSA, and HOS) and MCF7 cells (breast cancer cell line with an activating mutation in PIK3CA) were treated with (A) BEZ235 or (B) PIK75 or DMSO (control) for 24 h and viability was assessed with CellTiter-Glo. Experiments were conducted with eight replicates and displayed results are representative of three independent experiments. (C) Cells were treated with BEZ235 and PIK75 for 4 h and the effects on the PI3K/mTOR pathway were assessed. Cell extracts were analyzed by Western blotting with antibodies against phosphorylated AKT (Ser473), AKT, phosphorylated S6 (Ser235/236), S6, PARP, and GAPDH (loading control).