Table 5.
Anti-allergic activity of the ethanolic extract, fractions and pure constituents from Benchalokawichian remedy.
| Samples | %Inhibition at various concentrations | IC50 of Antiallergic activity | |||||
|---|---|---|---|---|---|---|---|
| Crude, semipure extracts, and pure compounds | 0.1 | 1 | 5 | 10 | 50 | 100 | IC50 ± SEM, μg/mL |
| (μM data for pure compound) | |||||||
| Ficus racemosa Linn. | — | 15.0 ± 10.5 | — | 28.9 ± 9.1 | 63.6 ± 1.6 | 75.8 ± 4.3 | 27.1 ± 1.6 |
| Capparis micracantha DC. | — | 4.2 ± 2.7 | — | 9.5 ± 7.1 | 22.9 ± 8.9 | 32.3 ± 6.3 | >100 |
| Tiliacora triandra Diels. | −24.48 ± 5.7 | −0.0 ± 1.8 | 11.7 ± 4.8 | 44.2 ± 4.6 | >100 | ||
| Harrisonia perforata Merr. | — | 21.6 ± 5.3 | — | 42.4 ± 1.3 | 62.8 ± 0.0 | 78.5 ± 0.8 | 14.5 ± 0.1 |
| Clerodendrum petasites S. Moore | −6.3 ± 5.1 | 7.9 ± 2.2 | 44.0 ± 1.4 | 85.0 ± 1.9 | 57.8 ± 1.4 | ||
| Benchalokawichian (Ha-Rak) | — | 9.7 ± 5.5 | — | 24.2 ± 3.6 | 52.1 ± 1.5 | 45.2 ± 5.1 | 39.8 ± 1.3 |
|
| |||||||
| Fraction 1; hexane (F1) | — | — | — | — | — | — | NT |
| Fraction 2; hexane : chloroform (F2) | — | 22.5 ± 0.4 | — | 30.4 ± 2.1 | 43.6 ± 0.8 | 53.0 ± 0.7 | 86.1 ± 1.1 |
| Fraction 3; chloroform (F3) | — | 23.1 ± 4.5 | — | 40.3 ± 3.4 | 59.5 ± 4.4 | 71.2 ± 1.7 | 17.9 ± 3.0 |
| Fraction 4; chloroform : methanol (F4) | — | 20.9 ± 7.7 | — | 38.9 ± 3.9 | 58.2 ± 2.7 | 70.5 ± 3.7 | 19.6 ± 3.4 |
| Fraction 5; methanol (F5) | — | 10.5 ± 7.6 | — | 26.0 ± 6.2 | 52.1 ± 1.5 | 63.9 ± 2.9 | 39.9 ± 1.9 |
|
| |||||||
| Compound 1; pectolinarigenin | 17.1 ± 1.7 | 25.1 ± 3.9 | 38.6 ± 7.2 | 101.1 ± 9.5 | — | — | 6.3 ± 0.7 (20.1 μM) |
| Compound 2; O-methylalloptaeroxylin | — | −15.8 ± 2.3 | 18.9 ± 0.9 | 40.5 ± 0.0 | — | 91.9 ± 1.6 | 14.2 ± 0.9 (51.8 μM) |
| Chlorpheniraminea | — | 4.0 ± 1.9 | — | 35.1 ± 0.8 | 44.4 ± 1.8 | 81.1 ± 3.2 | 16.2 ± 2.5 (58.8 μM) |
aPositive control.
NT (—) means not tested.