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. 2014 Nov 27;12:118. doi: 10.1186/1477-7827-12-118

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© Baddela et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effects of hypoxic and normoxic conditions and of different cell plating density on transcript abundance in cultured bovine granulosa cells. The expression of GAPDH (a), B2M (b), TBP (c), HMBS (d), RPS18 (e), RPLP0 (f) and HPRT1 (g) was analyzed under hypoxic (5% O₂) and normoxic conditions (20% O₂) at low and high cell plating density. Copy numbers were calculated and normalized to initial amounts of RNA used for cDNA preparation. Values are means ± SEMs of three independent cultures. Bars with different letters are significantly different from each other.