Table 2.

Real–time PCR conditions

Step	Description	Temperature	Time
1	Pre-incubation	95°C	5 min
2	Denaturation	95°C	20 sec
3	Annealing	60°C	15 sec
4	Extension	72°C	15 sec
5	Melting	95°C	5 sec
		70°C	60 sec
		97°C	1 s (continuous acquisition)