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. Author manuscript; available in PMC: 2014 Dec 31.
Published in final edited form as: J Immunol. 2009 Apr 15;182(8):4994–5002. doi: 10.4049/jimmunol.0803560

Figure 4. In situ hybridization of miR-21 in Aspergillus fumigatus challenged wild type mouse lung.

Figure 4

Expression of miR-21 in (A–B) Aspergillus fumigatus challenged wild type mouse lungs, were determined by LNA-based in situ hybridization. (A) LNA-anti-miR-21, left: 100X field; right: 400× field; (B) Left: 200× field, LNA-anti-miR-21. Right: serial sections at 600×; right top: LNA-anti-miR-21, right middle: anti-CD68, right bottom: LNA-scrambled control probe. (C) Relative expression of miR-21 in different cell types; BM DC: bone marrow derived dendritic cells; BM Mac: bone marrow derived macrophages; BALF: bronchoalveolar lavage fluid cells; MLE15: murine lung epithelial cell line; MFLM4: murine lung mensenchyme cell line; NIH 3T3: murine fibroblasts. (D) Relative expression of miR-21 in LPS stimulated murine macrophage cell line Raw264.7; ***, p < 0.001. Data are representative of three experiments.