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Validation of RPE senescence markers in vivo. (A) Immunofluorescent staining of methylated histone H3 foci on RPE flatmount prepared from Nrf2-deficient mice at either age 4 or 16 months. (B, C) Western blot analyses of amin B1 in RPE isolated from Nrf2-deficient (B) or WT mice (C) at the indicated age (*P < 0.05, Student's t-test).
