Figure 5.

Increased lysosome distribution of mTOR with RPE aging in vivo. Western blot analyses of LAMP1/2 and Rag-Ragulator complex proteins in RPE isolated from Nrf2-deficient mice (A) and wild-type mice at indicated ages (C). (B) Colocalization of mTOR (green) and LAMP1 (red) on immunofluorescent staining of RPE flatmount. Insets showed the enlarged field of interest. Pearson's correlation coefficient was determined to quantify the colocalization of signals. Scale bar: 20 μm. Data presented are representative of four separate experiments. *P < 0.05, **P < 0.01, Student's t-test.