Effects of CD40-TRAF blocking peptides on ICAM-1 upregulation in HREC stimulated with CD154. Three hours before the onset of stimulation with CD154, HREC were incubated with control, CD40–TRAF2,3, or CD40-TRAF6 blocking peptides (1 μM). Cells then were incubated with or without CD154 for 24 hours. The ICAM-1 expression was examined by flow cytometry. Results are shown as mean ± SEM and are representative of 3 experiments. *P < 0.05, represent comparison to cells treated with control peptide.