Table 4.

In vitro protein binding, species comparison

Concentration [μg/mL]	In vitro protein binding (%) mean, ± SEM
	Human [n = 4]	Dog [n = 3]	Rat [n = 3]
10	57 ± 3.0	56 ± 0.75	50 ± 1.1
1	59 ± 2.7	56 ± 0.75	50 ± 0.64
0.1	57 ± 2.5	57 ± 1.4	51 ± 0.35

In vitro protein binding using heparinized pooled plasma of 12-h-fasted male and female dogs (pool of three animals), rats (pool of five animals), and male human volunteers (individual plasma) (fasted for 10 h). Samples were spiked with [¹⁴C]-labeled imepitoin stock solution (concentration 1 mg/mL) to achieve a final drug concentration of 0.1, 1, or 10 μg/mL imepitoin in plasma. To allow protein binding, samples were incubated for 15 min prior to ultracentrifugation.