Effect of perchlorate on thyroid hormone levels. Mean concentrations of T4 and T3 in adult stickleback chronically exposed since fertilization to 0, 10, 30, or 100 ppm perchlorate over 336 days of development (error bars are ± 1 SE.). (A) Whole body thyroxine (T4, dashed line, left axis) and whole body triiodothyronine (T3, solid line, right axis) levels in adult stickleback chronically exposed to 0, 10, 30, or 100 ppm perchlorate. The asterisk (*) denotes significantly different [T3] from control. (B) Developmental T4 (top panels, dashed line) and T3 (bottom panels, solid line) concentrations in stickleback exposed to 0, 10, 30, or 100 ppm perchlorate for up to 336 days of development with ages grouped into category stages larval (up to 100 dpf), juvenile (100–250 dpf), and adult stickleback (older than 250 dpf), n = 5–18 pools (biological replicates) of age matched fish (mass equal to 1 g).