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Immunoblots of purified recombinant HuD reacted with the serum of a patient with small cell lung cancer and paraneoplastic limbic encephalitis. Each lane is a different sample of the same patient’s serum; the dilutions are (A) 1:50, (B) 1:100 and (C) 1:200. The serum of the patient was shown to be positive for anti-Hu antibody (dilution, 1:50–1:200).
