Figure 2.

AMG 139 inhibits IL-23 activity in NK cells. Human NK cells were incubated with native human IL-23 (A), recombinant human IL-23 (B), recombinant cynomolgus monkey IL-23 (C) or recombinant human IL-12 (D) in the presence of various concentrations of AMG 139 (A–D) or the positive control IL-12p35 antibody mAb219 (D). AMG 139 or mAb219 was incubated with IL-23 or 0.3 ng·mL⁻¹ IL-12, for 30 min prior to addition to the cells. After 24 h in culture, the supernatant was collected and IFN-γ was measured via elisa. Results are representative of at least three experiments, except (A) which is n = 2. Data are presented as mean ± SD.