Figure 5.

AtGLR3.5 knockout mutant plants show an accelerated senescence. A, Representative leaves from wild-type (wt) and knockout (atglr3.5-1) plants at 4, 5, and 6 weeks. B, The chlorophyll content in mutant leaves (n = 5) decreased faster than that of wild-type leaves 5 weeks after sowing. C, Increase in AtGLR3.5 gene expression during development. The results of three independent transcript analyses (±sd) obtained using densitometry are shown. D, Significant difference in SAG12 expression can be observed in the same plants (n = 3). The same results as shown here were obtained in another set of independent experiments. Left, representative gel of transcript analysis regarding SAG12 induction in wild-type and atglr3.5-1 plants at 4, 5, and 6 weeks. ACTIN transcript analysis was used as an internal control. A 100-bp DNA ladder was loaded. Right, graph showing statistically significant differences (asterisks) in SAG12 expression.