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. 2014 Dec 4;167(1):102–117. doi: 10.1104/pp.114.247767

Figure 3.

Figure 3.

Levels of auxin in roots of pdx1.1 and pdx1.3. A, GUS staining of leaves and roots of pdx1.1 and pdx1.3 compared with wild-type (WT) Col-0 carrying the auxin-responsive promoter DR5 fused to GUS. Seedlings were grown in the presence of 1% (w/v) Suc, and images were captured at 10 DAG. B, Quantitative GUS analysis performed with 4-methylumbelliferyl glucuronide (4-MU) in the absence (left) or presence (right) of Suc. Plant lines are at the same age as in A. The data are from at least three biological repetitions. Error bars represent se. Asterisks indicate statistically significant differences (P < 0.05) when compared with the wild type. C, Quantification of free and conjugated auxin in shoots and roots of pdx1.1 and pdx1.3 compared with the wild type. Seedlings were grown on medium in the presence of 1% (w/v) Suc and were analyzed at 10 DAG. The data are from at least three biological repetitions. Error bars represent se. Asterisks indicate statistically significant differences (P < 0.05) when compared with the wild type. FW, Fresh weight.