Table I. Kinetic parameters of EcCS and another E. coca BAHD acyltransferase, EcBAHD8.
Values preceding the ± sign represent sds from two technical replicates. N/A, Not available.
| Substrate | Apparent Km | Apparent kcat | kcat/Km | Apparent Ki |
|---|---|---|---|---|
| µm | s−1 | M−1 s−1 | mm | |
| EcCS | ||||
| Methylecgonine | 370 ± 26 | 9.7 ± 0.24 | 26,000 | N/A |
| Benzoyl-CoA | 93 ± 6.9 | 7.6 ± 0.17 | 82,000 | N/A |
| Methylecgonine | 62 ± 11 | 19 ± 1 | 306,000 | N/A |
| Cinnamoyl-CoA | 103 ± 8 | 46 ± 0.02 | 450,000 | N/A |
| EcBAHD8 | ||||
| Methylecgonine | 840 ± 64 | 0.02 ± 0.005 | 24 | N/A |
| Benzoyl-CoA | 6.9 ± 0.81 | 0.02 ± 0.004 | 2,900 | 5.5 ± 0.69 |
| Methylecgonine | 57 ± 6 | 0.003 ± 1.0 E-04 | 53 | N/A |
| Cinnamoyl-CoA | 93 ± 7 | 0.005 ± 1.1 E-04 | 54 | 14 ± 3 |