Figure 8.

mRNA expression and protein enrichment levels of β-carotene biosynthetic enzymes. A, mRNA expression of β-carotene biosynthetic enzymes in D. bardawil nitrogen-deprived (−N) cells. Enzyme names in black are located in chloroplast membranes; enzyme names in green are located in βC-plastoglobuli. Expression of 18S was added as a control. B, Protein enrichment levels. Numbers indicate the protein fold change increase in βC-plastoglobuli compared with chloroplast membranes (fold change calculations are described in “Materials and Methods”).