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. 2014 Nov 21;167(1):137–152. doi: 10.1104/pp.114.249268

Figure 1.

Figure 1.

Isolation and characterization of the ced2 mutant. A, ABA contents in ced2 and wild-type (WT) seedlings under the control or osmotic stress treatment. Two-week-old seedlings were treated with 40% (w/v) PEG solution for 6 h before ABA measurement. FW, Fresh weight. B, RNA gel-blot analysis of the expression of ABA biosynthesis genes in ced2 and wild-type plants in response to osmotic stress generated by the indicated concentrations of PEG. Ribosomal RNA (rRNA) was used as loading control. C, Morphology of seedlings on control or –0.7 MPa PEG-infused agar plates. Seeds were directly sowed on the shown plates, and pictures were taken 2 weeks after seed imbibition. D, Root growth (top) and shoot fresh weight (bottom) of wild-type and ced2 seedlings on MS agar plates with different osmotic potentials at 2 weeks after seed imbibition. Six plants were pooled together for shoot fresh weight measurement per experiment. Three replicates were performed. Data in A and D represent means ± se. *P < 0.05 and **P < 0.01.