Figure 6.
VSRs maintained membrane potential, calcium flux, and pHvac under osmotic stress. A, Time course of the relative membrane potential in roots of the wild type (WT) and ced2 in response to 40% (w/v) PEG treatment. B, Time course of the relative membrane potential in roots of the Col-0, vsr1/vsr3, and vsr1/vsr4 in response to 40% (w/v) PEG treatment. C, Calcium flux in roots of the wild type and ced2 in response to 40% (w/v) PEG treatment. D, Calcium flux in roots of the Col-0, vsr1/vsr3, and vsr1/vsr4 in response to 40% (w/v) PEG treatment. E, pHvac in root tips and mature roots of the wild type and ced2 with or without 40% (w/v) PEG treatment for 1 h. F, pHvac in roots of the Col-0, vsr1/vsr3, and vsr1/vsr4 in response to 40% (w/v) PEG treatment. For membrane potential and calcium flux assays, three independent experiments with 10 seedlings each were performed for each genotype. For pHvac measurements, three independent experiments were performed with 15 seedlings for each genotype. Data in A to F represent means ± se. *P < 0.05 and **P < 0.01. Col-0 indicates the wild type; ced2 indicates ced2 mutant; vsr1/vsr3 and vsr1/vsr4 indicate double mutants.