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. 2014 Nov 6;167(1):228–250. doi: 10.1104/pp.114.249946

Figure 5.

Figure 5.

Phenotypic growth analyses show defects in biomass production in aox1a rpoTmp double mutants. Phenotypic analysis was carried out according to the work by Boyes et al. (2001) to define growth stages for wild-type plants (Col-0) as well as aox1a-1, aox1a-2, rpoTmp-1, and rpoTmp-2 and double mutants aox1a-1 rpoTmp-2 and aox1a-2 rpoTmp-1. Measurements taken are the average of at least 10 plants grown on plate media (0% Suc). ses are shown. A, Growth stage progression timeline. Highlighted gray horizontal bars show individual growth stages. Time is shown on the x axis. B, Photographic representation at particular time points for the mutant lines. C, Number of rosette leaves over the course of development to 38 d for all plant lines. D, Root growth measures for all lines taken 12 d after sowing. Plants were grown on vertical plates (“Materials and Methods”), and growth analysis was repeated a minimum of three times. In the bar graph, different letters indicate statistically significant differences (P < 0.05). E, Soil-grown aox1a, rpoTmp, and aox1a rpoTmp plants of the same age show that the double mutants do not reach the size of rpoTmp plants, even after several weeks of growth. Aerial parts of aox1a plants were previously shown to match wild-type growth under normal conditions (Giraud et al., 2008). F, Green siliques for all genotypes were dissected to visualize embryo development. Red arrows indicate aborted ovules seen in double mutants. Silique length was measured at the completion of flowering in at least 15 siliques from >10 plants for all genotypes. ses are shown. In the bar graph, different letters indicate statistically significant differences (P < 0.05). G, Five and one-half-week-old aox1a rpoTmp plants grown on soil show yellowing of leaf tissue and lesions. H, In vivo respiratory rates measured in leaf discs from wild-type and aox1a:rpoTmp plants of the same developmental stage grown under normal conditions. rpoTmp-1 grown under the same conditions was included in this experiment for comparison. Oxygen consumption was additionally determined in the presence of the cytochrome oxidase inhibitor KCN. Error bars indicate sds (n = 3). FW, Fresh weight.