Figure 2.
BTS affects root iron response and embryogenesis. A, Relative BTS expression in root tissue from 7-d-old seedlings grown on +Fe medium for 4 d and then transferred to ±Fe medium for 3 d. Error bars indicate ± se of the mean (n = 4), and columns with different letters are significantly different from each other (P < 0.05). B, Root growth of 11-d-old seedlings grown on –Fe medium (4 d +Fe and 7 d –Fe). C, Rhizosphere acidification of 8-d-old seedlings grown on –Fe medium (4 d +Fe, 3 d –Fe, and 1 d bromocresol purple). Eight plants per genotype were grouped on bromocresol purple agar medium. Results shown represent four independent assays. D, Iron reductase activity of 10-d-old seedlings grown on ±Fe medium (7 d +Fe and 3 d ±Fe). Error bars indicate ± se of the mean (n = 4), and columns with different letters are significantly different from each other (P < 0.05). E, Embryo lethality in developing siliques. Error bars indicate ± se of the mean (n = 5). The asterisk indicates significant difference from the wild type (WT; P < 0.05). F, Iron and zinc content in seeds (150 mg per replicate) analyzed by ICP-OES. Error bars indicate ± se of the mean (n = 3), and columns with different letters are significantly different from each other (P < 0.05) G, Iron (Fe3+) localization in germinating seeds visualized by Perls staining. Results shown represent 10 independent assays. Bars = 2 mm. FW, Fresh weight.