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. 2014 Dec 1;167(1):273–286. doi: 10.1104/pp.114.250837

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Figure 4. — PYE and PYEL proteins form heterodimers in the nucleus. A, BiFC assay showing in planta interactions between PYE, ILR3, bHLH104, and bHLH115 proteins in the epidermal cells. Indicated proteins are transiently coexpressed in RFP-Histone2B (nuclear marker) transgenic N. benthamiana plants. EYFP fluorescence indicates interaction between indicated proteins. All interactions were tested using both combinations of N-/C-EYFP-fused reciprocal proteins. Results shown represent three independent assays. B, Immunoblot analysis showing Co-IP of in vitro-translated HA-tagged PYE, ILR3, or bHLH104 and Myc-tagged ILR3, bHLH104, and bHLH115 proteins. Results shown represent two independent assays. Bars = 20 μm.