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. 2014 Nov 6;167(1):176–188. doi: 10.1104/pp.114.251017

Figure 4.

Figure 4.

Purification of the recombinant SgMDH1 protein. A, SDS-PAGE analysis of SgMDH1 at different expression steps in E. coli. Lane 1, Molecular mass marker. Lane 2, Cells transformed with the empty vector pGEX-6P-3 without induction by 0.2 mm IPTG. Lane 3, Cells transformed with the empty vector pGEX-6P-3 induced by 0.2 mm IPTG. Lane 4, Cells transformed with the recombinant SgMDH1 protein without induction by 0.2 mm IPTG. Lane 5, Cells transformed with the recombinant SgMDH1 protein induced by 0.2 mm IPTG. B, SDS-PAGE analysis of SgMDH1 across purification steps in E. coli. Lane 1, Molecular mass marker. Lane 2, Total E. coli protein with the recombinant SgMDH1 protein induced by 0.2 mm IPTG. Lanes 3 to 5, Contaminate proteins at each of three steps of elution by binding buffer. Lane 6, Purified recombinant SgMDH1 protein after PreScission Protease incubation to cleave the GST tag. C, Western-blot analysis of purified recombinant SgMDH1.