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. 2014 Nov 6;167(1):176–188. doi: 10.1104/pp.114.251017

Table I. Kinetic constants and calculated kinetic parameters of the recombinant SgMDH1 protein.

Values represent the mean of three replicates with se.

Substrate Km Vmax Kcat/Km
mm nmol−1 min−1 mg protein−1 mm−1 min−1
OAA 0.158 ± 0.00 790.6 ± 21.4 475,000
NADH 0.302 ± 0.05 400.6 ± 44.4 126,017
Malate 1.230 ± 0.13 134.1 ± 10.0 10,357
NAD+ 0.240 ± 0.03 211.7 ± 6.44 83,798