Table I. Kinetic constants and calculated kinetic parameters of the recombinant SgMDH1 protein.
Values represent the mean of three replicates with se.
Substrate | Km | Vmax | Kcat/Km |
---|---|---|---|
mm | nmol−1 min−1 mg protein−1 | mm−1 min−1 | |
OAA | 0.158 ± 0.00 | 790.6 ± 21.4 | 475,000 |
NADH | 0.302 ± 0.05 | 400.6 ± 44.4 | 126,017 |
Malate | 1.230 ± 0.13 | 134.1 ± 10.0 | 10,357 |
NAD+ | 0.240 ± 0.03 | 211.7 ± 6.44 | 83,798 |