Table II. Effects of metal ions on the activity of the recombinant SgMDH1 protein.
SgMDH1 activity against 1 mm OAA was determined in reaction buffer supplied with or without the listed metal ions. Relative activity was expressed as the percentage of SgMDH1 activity without application of the metal ions. The relative activity value represents the mean of four replicates with se. Different letters in the same column indicate significant differences at the P = 0.05 level.
Metal Ion | Concentration | Relative Activity |
---|---|---|
mm | % | |
Mn2+ | 1.0 | 129.48 ± 7.72a |
Cu2+ | 1.0 | 105.66 ± 10.00b |
Mg2+ | 1.0 | 103.81 ± 7.71b |
Zn2+ | 1.0 | 88.17 ± 3.10b |
Al3+ | 1.0 | 87.13 ± 2.52b |
Fe2+ | 1.0 | 46.75 ± 2.27c |