Figure 6.

Quantitative analysis of FAs and glycerolipids in P. tricornutum grown in nutrient-replete conditions or in medium devoid of either N or P. Lipids from Pt1 cells grown either in a replete medium (10N10P; black) or in medium deprived of N (0N10P; blue) or P (10N0P; red) were extracted, separated by TLC, and quantified as described in “Materials and Methods.” To avoid any N deficiency in 10N10P or 10N0P culture, media were replaced by fresh ESAW 10N10P or 10N0P medium every 3 d. Lipids were analyzed after 5 d of cultivation in replete conditions (black bars), N starvation (red bars), or after 13 d for P starvation (blue bars). A, Changes in glycerolipid content. Note that in the P-depleted condition, phospholipids were not detectable. B, Changes in FA content. C, FA profile in TAG. Each result is the average of three biological replicates ± sd. ASQ, Acyl-SQDG; FFA, free FAs.