Table I. Identification of glycerolipids from P. tricornutum.
Characteristic fragments generated by fragmentation of the parent ion (MS2) are shown, together with the associated references.
| Analyzed Lipids | Polarity | Ion Analyzed | Specific Fragments in MS2 Scan | References |
|---|---|---|---|---|
| Phospholipids | ||||
| PC | + | [M + H]+ | Neutral loss of m/z 59 | Domingues et al. (1998) |
| PE | + | [M + H]+ | Neutral loss of 141 | Brügger et al. (1997) |
| Phosphatidylserine | + | [M + H]+ | Neutral loss of 185 | Brügger et al. (1997) |
| PG | + | [M + NH4]+ | Neutral loss of 189 | Taguchi et al. (2005) |
| PI | + | [M – H]– | Precursors of m/z 241 | Hsu and Turk (2000b) |
| Phosphatidic acid | + | [M + NH4]+ | Neutral loss of 115 | Li-Beisson et al. (2010) |
| Nonphosphorus glycerolipids | ||||
| SQDG | – | [M – H]– | Precursors of m/z 225 | Gage et al. (1992); Welti et al. (2003) |
| ASQ | – | [M – H]– | Precursors of m/z 509 | Naumann et al. (2011) |
| MGDG | + | [M + NH4]+ | Neutral loss of 179 | Li-Beisson et al. (2010) |
| DGDG | + | [M + NH4]+ | Neutral loss of 341 | Moreau et al. (2008) |
| DGTA | + | [M + H]+ | Precursors of m/z 236; neutral loss of 59; no neutral loss of 87 as found for DGTS | Armada et al. (2013) |
| Neutral glycerolipids | ||||
| Free FA | – | [M – H]– | ||
| DAG | + | [M + NH4]+ | Scan of [M+NH4-RCOONH4]+ | Camera et al. (2010) |
| TAG | + | [M + NH4]+ | Scan of [M+NH4-RCOONH4]+ | Hsu and Turk (2010) |