Table I. Identification of glycerolipids from P. tricornutum.
Analyzed Lipids | Polarity | Ion Analyzed | Specific Fragments in MS2 Scan | References |
---|---|---|---|---|
Phospholipids | ||||
PC | + | [M + H]+ | Neutral loss of m/z 59 | Domingues et al. (1998) |
PE | + | [M + H]+ | Neutral loss of 141 | Brügger et al. (1997) |
Phosphatidylserine | + | [M + H]+ | Neutral loss of 185 | Brügger et al. (1997) |
PG | + | [M + NH4]+ | Neutral loss of 189 | Taguchi et al. (2005) |
PI | + | [M – H]– | Precursors of m/z 241 | Hsu and Turk (2000b) |
Phosphatidic acid | + | [M + NH4]+ | Neutral loss of 115 | Li-Beisson et al. (2010) |
Nonphosphorus glycerolipids | ||||
SQDG | – | [M – H]– | Precursors of m/z 225 | Gage et al. (1992); Welti et al. (2003) |
ASQ | – | [M – H]– | Precursors of m/z 509 | Naumann et al. (2011) |
MGDG | + | [M + NH4]+ | Neutral loss of 179 | Li-Beisson et al. (2010) |
DGDG | + | [M + NH4]+ | Neutral loss of 341 | Moreau et al. (2008) |
DGTA | + | [M + H]+ | Precursors of m/z 236; neutral loss of 59; no neutral loss of 87 as found for DGTS | Armada et al. (2013) |
Neutral glycerolipids | ||||
Free FA | – | [M – H]– | ||
DAG | + | [M + NH4]+ | Scan of [M+NH4-RCOONH4]+ | Camera et al. (2010) |
TAG | + | [M + NH4]+ | Scan of [M+NH4-RCOONH4]+ | Hsu and Turk (2010) |