Figure 1.

Expression of PsCRN63 or PsCRN63/PsCRN115 in planta enhanced the susceptibility of N. benthamiana to P. capsici. A, Leaf phenotypes upon P. capsici infection. A. tumefaciens was used to transiently express the indicated CRN effectors (PsCRN63 or PsCRN115 alone or coexpressing both effectors) as GFP fusions on one-half of an N. benthamiana leaf and free GFP on the other. Leaves were infected with P. capsici zoospores 12 h after infiltration. Typical photographs were taken 24 h after inoculation under normal light (left) and UV light (right). B, Nuclear localization of GFP florescence signals in the infiltrated leaves. The infiltrated leaves were detached at 24 h after infiltration, and GFP fluorescence was detected using a confocal laser scanning microscope. C, Average lesion diameters of N. benthamiana leaves expressing CRN effectors or GFP. Error bars represent se calculated from at least 11 independent biological replicates. The letters indicate significant differences (P < 0.01) determined by a nonparametric Kruskal-Wallis test.