Figure 5. PCR screening of multi-fragment assembled clones from Table 3.

Eight clones (colonies) from each construct were grown overnight in the 96-well plate containing LB + Spectinomycin (50 ug/ml). One µl of the over-night grown cell culture was used for PCR screening with base vector primers (18 bp) located immediately upstream of the promoter and downstream of the terminator. PCR products for each construct were loaded every other lane on a 1.0% agarose gel (containing ethidium bromide) with a multi channel pipette. GeneRuler 1 kb Plus DNA Ladder was used as the DNA marker.