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. 2014 Dec 31;9(12):e115318. doi: 10.1371/journal.pone.0115318

Figure 8. Comparison study of Hot Fusion and Gibson Assembly (NEB kit).

Figure 8

(A) Cloning strategy for single and multi-fragment assembly. A full lacZ gene and three pieces of the lacZ gene with overlapping sequences were amplified, and cloned into a base vector digested with SfaAI (unpurified). (B) Transformation efficiency of lacZ assembly by Hot Fusion and Gibson Assembly (from NEB). The top panels are the transformation plates from Hot Fusion. The bottom panels are the transformation plates from Gibson Assembly. The same amount of base vector was used in all reactions and the same amount of insert was used in the same treatment. Blue colonies contain the lacZ gene or all three pieces of the lacZ gene correctly cloned or assembled together (in frame). White colonies contain the parent vector. (C) Cloning efficiency of Hot Fusion and Gibson Assembly. Colonies were counted from each transformation plate.