Table 1. Examples of the oligo primers used in this study.

Primer name	Sequences (5' ->3')	Application
Mon-For1	GCGCTGTGCCTGTTGCGAT CGCACCGGATCTCTATAATCTCGCGCAACC	single fragment cloning
Mon-Rev1	GCGCGTGTTCTGCTGGCGAT CGCGGAATAATAGCGAGAACAGAGAAATAGC
ICD-For1	AAAAGAGATGGAGGAAGAAGNNNN(24)	Single fragment cloning
ICD-Rev1	CTGAATTCTGAGAGCAAGNNNN(24)
lacZ-Frag1-For1(1)	GGTACCTCAGCGCTGTGCCTGTTGCG ATACCGGATCTCTATAATCTCGCGCAACC	Multi-fragment cloning
lacZ-Frag1-Rev1(420)	CGCAACTGTTGGGAAGGGCGATCGGTG
lacZ-Frag2-For2(394)	CACCGATCGCCCTTCCCAACAGTTGCG	Multi-fragment cloning
lacZ-Frag2-Rev2(2388)	CATGCGGTCGCGTTCGGTTGCACTAC
lacZ-Frag3-For3(2362)	GTAGTGCAACCGAACGCGACCGCATG	Multi-fragment cloning
lacZ-Frag3-Rev3(3566)	AACCTCAGCGCGTGTTCTGCTGGCG ATGGAATAATAGCGAGAACAGAGAAATAG

Underlined nucleotides indicate the overlapping sequences with the adjacent fragment or vector to be joined.