Table 2. PCR screening of colonies from nine Hot Fusion transformation plates.
| Plate # | PCR product intensity | Total white colonies | Total blue colonies | Colonies screened (white) | Contained insert | Positive clones (with correct insert size) | Efficiency (%) |
| A1 | - | 14 | 4 | 8 | 0 | 0 | 0 |
| B1 | ++++ | >1,000 | 3 | 8 | 8 | 8 | 100 |
| C1 | ++++ | >1,000 | 5 | 8 | 8 | 8 | 100 |
| D1 | ++++ | >600 | 3 | 8 | 7 | 7 | 88 |
| E1 | ++++ | >800 | 2 | 8 | 8 | 7 | 88 |
| F1 | + | ∼200 | 3 | 8 | 8 | 3 | 38 |
| G1 | ++++ | ∼250 | 4 | 8 | 8 | 4 | 50 |
| H1 | - | 12 | 2 | 8 | 0 | 0 | 0 |
| A2 | ++++ | >1,000 | 13 | 8 | 8 | 8 | 100 |
Eight white colonies were screened for each construct. Non-purified PCR products were used in this study. Efficiency indicates % of clones that contained the expected insert.