Table 2.
Antibodies used in immunofluorescence analysis with the Tyramide Signal Amplification (TSATM) method
| Primary antibody | Species | Source | Dilutiona | Detection method | Source | Dilution |
|---|---|---|---|---|---|---|
| PYY (aa 4–21) | Rabbit | Biosource | 1:1,000,000 | HRP-conjugated donkey anti-rabbit TSATM-Plus Cy5 |
Jackson ImmunoResearch PerkinElmer |
1:1,000 1:200b |
| GLP-1 | Rabbit | Abcam | 1:500 | Cy3-conjugated donkey anti-rabbit | Jackson ImmunoResearch | 1:1,000 |
| GFP | Chicken | Abcam | 1:1,000 | Dylight488-conjugated donkey anti-rabbit | Jackson ImmunoResearch | 1:1,000 |
PYY peptide tyrosine tyrosine, GLP-1 glucagon-like peptide 1, GFP green fluorescent protein
a
All antibodies except for TSATM were diluted in 50 mM TRIS-buffered saline containing 0.05% Tween-20 and 0.1% bovine serum albumen
b
TSATM-Cy5 reagent was diluted in 1X Plus Amplification Diluent provided with the TSATM kit (PerkinElmer, Waltham, MA)