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. 2014 Dec 31;9(12):e116056. doi: 10.1371/journal.pone.0116056

Table 2. Candidate gene list and their primers for quantitative real time-PCR.

Name Tamplate ID Genome code of Citrus sinensis Similarity (e-value) Annotation Primer sequence 5′ to 3′
Abscisic acid metabolism and signal transduction pathway
CsNCED1 All-Unigene9577 Cs5g14370 0 9-cis-epoxycarotenoid dioxygenase [EC: 1.13.11.51] Forward: AACCCGTCTGCCAGAACCTT
Reverse: GTTGGCTCCGTTTCTGACGTA
CsNCED3 All-Unigene111 Cs6g19500 3.58E-07 9-cis-epoxycarotenoid dioxygenase [EC: 1.13.11.51] Forward: GCTTCCGTTTGTGGCCTACTT
Reverse: ATTGACCCGGCATTTTTATGTG
CsAAO All-Unigene40211 Cs8g13770 7.76E-15 abscisic-aldehyde oxidase [EC: 1.2.3.14] Forward: CGCATGCGTTGTCCTACTGT
Reverse: AAGACCTTCGCTTGTGGTAATTG
CsABA8ox1 All-Unigene16990 Cs6g19380 5.00E-132 ()-abscisic acid 8′-hydroxylase [EC: 1.14.13.93] Forward: GCCCAAAAGTCAAAGGACAAGT
Reverse: CATCATCATTTCGGCTTTCCA
CsABA8ox3 All-Unigene8405 Cs1g09250 4.03E-03 ()-abscisic acid 8′-hydroxylase [EC: 1.14.13.93] Forward: TTAAGAATGGAACCGCCGAAT
Reverse: TTGGGAATGGTGTATCCATCAA
CsABI1 All-Unigene41252 Cs4g20430 0 ABA-insensitive 1; K01090 protein phosphatase [EC: 3.1.3.16] Forward: GCCTCCTCCAAACTTGATTGC
Reverse: CCCTCAAACCCTCAGCAGAA
CsAHG1 All-Unigene1776 Cs9g16360.1 1.36E-02 ABA-Hypersensitive germination1; K01090 protein phosphatase [EC: 3.1.3.16] Forward: GGGCCTCGGATGGTAGAAGA
Reverse: AGCAAGCCGGGTTAACAATG
CsAHG3 All-Unigene15798 Cs7g31880.1 9.04E-11 ABA-Hypersensitive germination3 Forward: GCTAGAGCTCCGTCCGTTTAAC
Reverse: GCTTCTTTCGTTTCCGATCGT
CsHAB1 All-Unigene40938 Cs1g17890.9 5.73E-03 Hypersensitive to ABA1; K14497 protein phosphatase 2C [EC: 3.1.3.16] Forward: GGCAAGGTCATCCAATGGAA
Reverse: AGCAGAGAAATAACACAATGTCAAAGA
CsHAB2 All-Unigene40938 Cs1g17890.4 5.73E-03 Hypersensitive to ABA2; K14497 protein phosphatase 2C [EC: 3.1.3.16] Forward: CACTGTGCAATGCCTAGTCAGTATAC
Reverse: TGTGGCGGCAGACAGTCAT
CsHAI1 All-Unigene8244 Cs8g19140.1 3.10E-06 Highly ABA-Induced1 Forward: CACGTTTGTCCCCACCAGAT
Reverse: CGTTGGCTTGCTGCTGCTA
CsPYL4 All-Unigene756 Cs7g30500.1 3.18E-123 PYR1-like protein 4; K14496 abscisic acid receptor PYR/PYL family Forward: GGAAACTTGCACTTTTGTTGAGACT
Reverse: TAGCAGCCATGTTCTCCGAAA