Figure 8. Reusability of mCLEAs of CALB at different temperatures.

Biocatalyst was used in consecutive reaction cycles of 24 h for biodiesel (FAPEs) conversion from olive oil at: (a) 30°C, (b) 40°C, (c) 50°C and (d) 60°C. The reaction mixture and conditions were the same as in Fig. 6. Relative conversion is referred to the final conversion after the first cycle and was calculated using samples analyzed by HPLC. Two types of mCLEAs were tested which were prepared using 0.05 (◯) and 0.1 mg protein/mg MNP-NH₂ (•).