Table 2. Effect of cross-linker concentration on the preparation of mCLEAs of CALB.

Glutaraldehyde (mM)	Recovered activity (U/mg MNP–NH2)	Apparent retained activity (%)
0	0.021	1.1
5	0.019	1.0
10	0.031	1.6
25	0.079	4.1
75	0.391	20.5
125	0.893	46.8
200	1.238	64.8
250	1.188	62.2
300	1.180	61.8
350	1.285	67.3
400	1.315	68.8
500	1.142	59.8