Figure 4. FtsA effects on sedimentation characteristics of FtsZ from Deinococcus radiodurans and Escherichia coli.
Purified recombinant Dr-FtsZ (A) and Ec-FtsZ (B,) were incubated in different combinations with GTP, ATP, Dr-FtsA (Dr) and Ec-FtsA (Ec) and sedimentation characteristics of FtsZs were monitored as described in methods. Both pellets and supernatant fractions were analysed on SDS-PAGE and coomassie stained protein bands were quantified densitometrically and depicted in bar diagrams (C, D). Sample identity (1–6) in panel C and D is similar to panel A and B, respectively.