Figure 7. In vivo interaction of FtsA with FtsZ using bacterial two hybrid system.

Dr-ftsA, Ec-ftsA, Dr-ftsZ and Ec-ftsZ were cloned in bacterial two hybrid system plasmids as described in methods and given in Table 2. These were cotransformed into E. coli BTH101 in different combinations. Expression of β-galactosidase was assayed in liquid culture of these cells grown overnight in presence of IPTG and activity calculated as described in methods. Cells co-transformed with both the vectors (KNT25+UT18C), with one vector and one construct (KNT25+Ec-FtsA) were used as negative control while CheA subunit expressing on both the vectors (CheA+CheA) was used as positive control.