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. 2014 Dec 31;9(12):e115918. doi: 10.1371/journal.pone.0115918

Figure 8. Effect of Dr-FtsA and Dr-FtsZ over expression on cell division in Escherichia coli.

Figure 8

E. coli MG1655 was transformed with vectors pBADHisA and pVHSGFP (HisA+GFP), pBADHisA and pFTSZGFP (HisA+FtsZ), pBADDftsA and pVHSgfp (FtsA+Gfp), and pBADftsA and pFTSZGFP (FtsA+FtsZ). These cells were grown in the absence (1) and presence of IPTG (2), arabinose (3) and both IPTG and arabinose (4) for 3 h. These cells were micrographed (A) and cell lengths of nearly 500 independent cells were measured and presented as histograms (B). Aliquots were taken and nearly equal amount of total proteins were analysed on SDS-PAGE (SDS-PAGE) and blotted with antibodies against (His)6 tag (His-FtsA) and Dr-FtsZ(GFP-FtsZ) (C).