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. 2014 Dec 31;9(12):e116039. doi: 10.1371/journal.pone.0116039

Figure 3. cpn60-targeted fluorescent microsphere hybridization assay to detect ‘Ca.Phytoplasma’ spp.

Figure 3

Results are shown for a 4-plex assay (format used for analysis of 192 B. napus DNA extracts) on plasmid DNA controls (107 copies/PCR) and on genomic DNA extracted from various infected plant tissues. Beads with a positive hybridization signal in each sample are identified (*). Samples from infected plant tissues are those described in S1 Table (onion, item #25; flax, item #41; vinca1, item #33; vinca2, item #35; carrot, item #15). Abbreviations: MFI, median fluorescence intensity; BN, Bois Noir; ESFY, European Stone Fruit Yellows; AY, Aster Yellows.