Table 3. Summary of diagnostic assays conducted on 192 field-collected samples of B. napus from the 2012 growing season.

			samples with typing results
test	total pos	total neg	AY-OY-M	AY-WB	AY-OM and AY-WB	Totalb
cpn60 PCR-gel electrophoresisa	153	39	27	11	0	38
cpn60 PCR-fluorescent microsphere assaya	125	67	53	68	4	125
16S–23S PCR-gel electrophoresis	123	69	NDc	ND	ND	ND

Complete results for each sample are shown in S3 Table.

^aassayed using H279p/H280p.

^bonly PCR products from samples that gave discordant results between P1/Tint (neg) and H279p/H280p (pos) were directly sequenced. One reaction failed so no typing information is available. For the fluorescent microsphere assays, all positive samples returned a typing result.

^cND, not determined.