Figure 3. ANS binding assay (left panel) of lysozyme (A), RNase-A (B) and α-LA (C) treated overnight at 37°C with varying concentrations of HTL.

Right panels depict λ_max as a function of HTL concentrations of lysozyme (D), RNase-A (E) and α-LA (F). To maintain clarity, only the representative curves of free dyes (dotted lines), control unmodified protein (solid lines) and protein modified with 1000 µM HTL (dashed lines) are shown.