Table 4. Enzymatic activity parameters (K _m and k _cat) of lysozyme and RNase-A treated with different concentrations of HTL^a.

	Lysozyme		RNase-A
HTL (µM)	K m (µg ml−1)	k cat (mg ml−1 s−1M−1)	K m (mM)	k cat (min−1)
0	77.8±2	484.1±29	1.29±0.11	191±22
50	76.9±4	489.3±25	1.33±0.17	185±23
100	78.1±3	479.9±27	1.27±0.09	192±25
200	77.4±4	481.3±31	1.28±0.13	195±28
400	77.6±3	478.8±26	1.29±0.12	194±23
600	77.1±2	482.3±24	1.31±0.14	196±27
800	78.3±3	484.5±28	1.32±0.16	189±22
1000	77.3±4	481.2±31	1.25±0.13	198±24

^a± represents the average errors of three independent measurements.